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Rashmi ThakurResearch Scholar, LPU
Year four of the PhD

Rashmi Thakur

Editing finger millet for drought tolerance

Biotechnology research scholar at Lovely Professional University. My work runs between a tissue-culture hood and a terminal: I edit stress-response genes in finger millet, then read what shifts across thousands of transcripts. The point is a millet that still yields when the monsoon fails.

Portrait of Rashmi Thakur
0peer-reviewed papers
0citations
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What the work looks like

Finger millet is stubborn in the best way. It grows on thin soil, tolerates heat that flattens rice, and has been eaten across India for four thousand years. It is also badly under-studied, which means almost every experiment tells you something nobody has written down yet.

I spend most mornings in tissue culture and most afternoons in RStudio. The through-line is a single question: which regulatory genes decide whether a seedling survives drought and salt together, rather than one at a time? Combined stress behaves nothing like the sum of its parts, and that gap is where my thesis lives.

Alongside the editing work, I maintain the lab's transformation protocols, run the qPCR bench, and supervise two masters students through their dissertation projects. I read broadly outside my area — nanocarrier chemistry, soil microbiology, agricultural policy — because the useful ideas usually arrive from next door.

A cultivar that survives a failed monsoon is worth more than a perfect result in a growth chamber.
0Peer-reviewed publications
0Citations across all work
0h-index, Google Scholar
0Conferences presented at
supervision Dr. A. K. Sharma School of Bioengineering and Biosciences,
Lovely Professional University, Phagwara, Punjab
funding DST-INSPIRE Senior Research Fellowship Department of Science and Technology, Government of India

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Four threads, running at once

The thesis is one question approached from four directions. Each thread feeds the others — an editing result sends me back to the transcriptome, and a transcript pattern decides what gets edited next.

thread 01

Editing the millet genome

Multiplexed CRISPR–Cas9 knockouts and base edits across the DREB and NAC families, screened for seedlings that hold turgor under combined drought and salt.

CRISPR-Cas9base editingRNP deliveryguide design
thread 02

Reading the stress response

RNA-seq of seedlings under drought, salinity, and both together. Co-expression networks pick out the regulators that only appear when the two stresses arrive at the same time.

RNA-seqDESeq2WGCNAqRT-PCR
thread 03

Getting the cargo inside

Chitosan–ZnO nanocarriers that walk Cas9 ribonucleoproteins into seedlings directly, so editing no longer depends on a slow and genotype-fussy tissue-culture step.

chitosan-ZnOseedling uptakecytotoxicity
thread 04

Help from the rhizosphere

Bacillus and Pseudomonas endophytes isolated from arid Rajasthan soils, used to bio-prime seed. A low-cost intervention that works on the same trait from outside the genome.

endophytesbio-primingarid soils16S

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What I can run at the bench

Loaded as a gel, because that is how I think about it. The further a band has migrated, the more hours that technique has actually had from me.

lanes 1–8 — techniques, stained for time spent

Hover any lane for where I'd place myself. Everything here I have run unsupervised on my own samples, not just watched in a workshop.


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Published work

output by year
20222023202420252026
2026

Multiplexed CRISPR–Cas9 editing of DREB2A and NAC67 improves combined drought and salinity tolerance in finger millet

Thakur R, Sharma A K, Bedi N, Kaur H

Plant Cell Reports, 45(3), 411–428

journal article impact factor 6.0 18 citations read the paper
2025

Chitosan–ZnO nanocarriers deliver Cas9 ribonucleoproteins into Eleusine coracana seedlings without tissue culture

Thakur R, Verma S, Sharma A K

ACS Agricultural Science & Technology, 5(9), 1204–1216

journal article 34 citations read the paper
2025

Genome editing for climate-resilient millets: progress, bottlenecks and the Indian regulatory outlook

Thakur R, Sharma A K

Critical Reviews in Biotechnology, 45(2), 188–207

review 96 citations read the paper
2024

Comparative transcriptome profiling of finger millet under combined salinity and heat stress

Thakur R, Kaur H, Singh M, Sharma A K

Frontiers in Plant Science, 15, 1398221

journal article 71 citations read the paper
2024

Tissue-culture-free editing in small millets: a nanocarrier route

Thakur R, Sharma A K

International Plant Biotechnology Congress, New Delhi — oral presentation

conference best poster award see the abstract
2024

Bio-priming with arid-soil Bacillus endophytes sustains seedling vigour under water deficit

Bedi N, Thakur R, Sharma A K

Microbiological Research, 284, 127712

journal article 52 citations read the paper
2023

Abiotic stress regulons in small millets

Thakur R, Sharma A K

In Advances in Millet Biotechnology, Springer Nature, pp. 143–171

book chapter 23 citations see the chapter

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How I got here

2022 — present

PhD in Biotechnology

School of Bioengineering and Biosciences, Lovely Professional University

Thesis on multiplexed editing of DREB and NAC regulons for combined drought and salinity tolerance in finger millet. Coursework in advanced molecular biology, bioinformatics and research ethics, cleared with distinction.

2024 — present

Senior Research Fellow, DST-INSPIRE

Department of Science and Technology, Government of India

Own the lab's editing and transformation pipeline end to end. Mentor two masters students through their dissertations and run the weekly journal club.

2020 — 2022

M.Sc. Biotechnology

Lovely Professional University, Phagwara

University gold medal. Dissertation on endophytic bacteria from arid Rajasthan soils and their effect on seedling vigour — the work that pulled me toward stress biology.

summer 2021

Research intern

CSIR–Institute of Himalayan Bioresource Technology, Palampur

Ten weeks on plant tissue culture and secondary metabolite extraction. First time I ran a transformation on my own, and the first time I broke one.

2017 — 2020

B.Sc. Biotechnology (Hons.)

University of Rajasthan, Jaipur

Graduated first class. Final-year project on drought response in pearl millet, supervised by the plant physiology group.


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Funding and recognition

2024

DST-INSPIRE Senior Research Fellowship

Department of Science and Technology, Government of India

2025

Young Researcher Award

Indian Society of Plant Biotechnology, annual meeting

2024

Best poster, plant genome editing track

International Plant Biotechnology Congress, New Delhi

2022

University gold medal, M.Sc. Biotechnology

Lovely Professional University

2025

Two patent applications filed

Nanocarrier-mediated RNP delivery in cereal seedlings

2025

SERB international travel grant

Science and Engineering Research Board


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Get in touch

Open to collaborations on millet genomics, editing protocols and stress phenotyping, and to postdoctoral conversations for 2027 onward.

This form needs a backend before it can send. Until then, email is fastest.

where to find me Block 32, School of Bioengineering and Biosciences Lovely Professional University, Jalandhar–Delhi GT Road,
Phagwara, Punjab 144411, India