Editing the millet genome
Multiplexed CRISPR–Cas9 knockouts and base edits across the DREB and NAC families, screened for seedlings that hold turgor under combined drought and salt.
running gel — 0%
Editing finger millet for drought tolerance
Biotechnology research scholar at Lovely Professional University. My work runs between a tissue-culture hood and a terminal: I edit stress-response genes in finger millet, then read what shifts across thousands of transcripts. The point is a millet that still yields when the monsoon fails.
Finger millet is stubborn in the best way. It grows on thin soil, tolerates heat that flattens rice, and has been eaten across India for four thousand years. It is also badly under-studied, which means almost every experiment tells you something nobody has written down yet.
I spend most mornings in tissue culture and most afternoons in RStudio. The through-line is a single question: which regulatory genes decide whether a seedling survives drought and salt together, rather than one at a time? Combined stress behaves nothing like the sum of its parts, and that gap is where my thesis lives.
Alongside the editing work, I maintain the lab's transformation protocols, run the qPCR bench, and supervise two masters students through their dissertation projects. I read broadly outside my area — nanocarrier chemistry, soil microbiology, agricultural policy — because the useful ideas usually arrive from next door.
A cultivar that survives a failed monsoon is worth more than a perfect result in a growth chamber.
The thesis is one question approached from four directions. Each thread feeds the others — an editing result sends me back to the transcriptome, and a transcript pattern decides what gets edited next.
Multiplexed CRISPR–Cas9 knockouts and base edits across the DREB and NAC families, screened for seedlings that hold turgor under combined drought and salt.
RNA-seq of seedlings under drought, salinity, and both together. Co-expression networks pick out the regulators that only appear when the two stresses arrive at the same time.
Chitosan–ZnO nanocarriers that walk Cas9 ribonucleoproteins into seedlings directly, so editing no longer depends on a slow and genotype-fussy tissue-culture step.
Bacillus and Pseudomonas endophytes isolated from arid Rajasthan soils, used to bio-prime seed. A low-cost intervention that works on the same trait from outside the genome.
Loaded as a gel, because that is how I think about it. The further a band has migrated, the more hours that technique has actually had from me.
Hover any lane for where I'd place myself. Everything here I have run unsupervised on my own samples, not just watched in a workshop.
Plant Cell Reports, 45(3), 411–428
ACS Agricultural Science & Technology, 5(9), 1204–1216
Critical Reviews in Biotechnology, 45(2), 188–207
Frontiers in Plant Science, 15, 1398221
International Plant Biotechnology Congress, New Delhi — oral presentation
Microbiological Research, 284, 127712
In Advances in Millet Biotechnology, Springer Nature, pp. 143–171
School of Bioengineering and Biosciences, Lovely Professional University
Thesis on multiplexed editing of DREB and NAC regulons for combined drought and salinity tolerance in finger millet. Coursework in advanced molecular biology, bioinformatics and research ethics, cleared with distinction.
Department of Science and Technology, Government of India
Own the lab's editing and transformation pipeline end to end. Mentor two masters students through their dissertations and run the weekly journal club.
Lovely Professional University, Phagwara
University gold medal. Dissertation on endophytic bacteria from arid Rajasthan soils and their effect on seedling vigour — the work that pulled me toward stress biology.
CSIR–Institute of Himalayan Bioresource Technology, Palampur
Ten weeks on plant tissue culture and secondary metabolite extraction. First time I ran a transformation on my own, and the first time I broke one.
University of Rajasthan, Jaipur
Graduated first class. Final-year project on drought response in pearl millet, supervised by the plant physiology group.
Department of Science and Technology, Government of India
Indian Society of Plant Biotechnology, annual meeting
International Plant Biotechnology Congress, New Delhi
Lovely Professional University
Nanocarrier-mediated RNP delivery in cereal seedlings
Science and Engineering Research Board
Open to collaborations on millet genomics, editing protocols and stress phenotyping, and to postdoctoral conversations for 2027 onward.